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Full Instrument - Valve Control

High throughput single cell sorting directly from the Petri dish
One single cell arrives to each PCR tube
10 PCR strips containing 80 tubes can be filled in a cycle
Glass cover slip for testing single cell deposition in situ
Drop volume less than 1 ul for adherent cells
Pick up volume of ~1 nl for suspended cells
15-20 seconds per cell. When collecting multiple cells, sorting speed is 1 cell/second.
Number of cells picked up in a single run: 1-1000.
Isolates a subpopulation of live adherent cells expressing fluorescent or luminescent markers
Both unlabeled and fluorescentcells are recognized by computer vision
Viable cells after sorting
Any adherent and non-adherent cell type can be sorted
Cell culture needs minimal preparation before sorting
Average sorting process takes only a few minutes
Multichannel detection using the fluorescent filter setup of the microscope

Components

Valve Controlled Cellsorter Device & Software

High precision micropipette holding console made from hard aluminum equipped with LED illumination for micropipette focusing connected to the motorized micromanipulator via a rotatable arm.
25 mm travel range 1D motorized micromanipulator
Special aluminum insert fitting to the microscope stage with a hole and magnetic fixing ring for the 35 mm Petri dish and 80 holes for 10 PCR stripes and a hole for a 32x24 mm2 glass cover slide for testing and high resolution imaging and a hole with a calibration cross-hair
CellSorter control unit with 2 high speed fluid valves and USB connection to the computer
User friendly intuitive control software for MS Windows OS
Accessories, fittings and tubing for liquid control
5 pcs of borosilicate micropipettes for single cell manipulations with calibrated aperture

Motorized Zeiss Axio Observer A1 Inverted Fluorescent Microscope

Including NA 0.55 LD condensor lens, EC Plan neofluar 10x/0.3 Ph 1 objective lens
Accessories for phase contrast and fluorescent imaging
Fluorescent illumination system with Prior Lumen 200S metal halide light source
Marzhauser 2D motorized microscope stage and motorized focus drive.

Andor Zyla 5.5 Camera

USB 3.0 sCMOS – Compact low light imaging camera
Front-illuminated scientific CMOS sensor
2560 x 2160 pixel array, 6.5 μm pixel size
Low read noise of 1.2 e-, sensor stably cooled to 0°C up to an ambient temperature of +35°C
Rolling shutter and ‘true’ global shutter modes
12 bit and 16 bit digitization
Max. pixel readout rate 2x280MHz
iCam, C-mount, USB3.0 interface (interface card and cable included), power supply, trigger cable

NE-1000 Syringe Pump

Holds 1 Syringe up to 60 cc
Infusion rates from 0.73 µL/hr (1 cc syringe) to 2100 ml/hr (60 cc syringe)
Easy-to-use keypad interface
Space Saving Chassis: Foot print size of only 5 3/4" x 8 3/4"
Includes all the advanced functions and programming features of the NE-1000 family

Dell Desktop Computer With 23" Dell Monitor