Full CellSorter Instrument with Valve Control

  • High throughput single cell sorting directly from the Petri dish

  • One single cell arrives to each PCR tube

  • 10 PCR strips containing 80 tubes can be filled in a cycle

  • Glass cover slip for testing single cell deposition in situ

  • Drop volume less than 1 ul for adherent cells

  • Pick up volume of ~1 nl for suspended cells

  • 15-20 seconds per cell. When collecting multiple cells, sorting speed is 1 cell/second.

  • Number of cells picked up in a single run: 1-1000.

  • Isolates a subpopulation of live adherent cells expressing fluorescent or luminescent markers

  • Both unlabeled and fluorescentcells are recognized by computer vision

  • Viable cells after sorting

  • Any adherent and non-adherent cell type can be sorted

  • Cell culture needs minimal preparation before sorting

  • Average sorting process takes only a few minutes

  • Multichannel detection using the fluorescent filter setup of the microscope

Components

Valve Controlled Cellsorter Device & Software

 

  • High precision micropipette holding console made from hard aluminum equipped with LED illumination for micropipette focusing connected to the motorized micromanipulator via a rotatable arm.

  • 25 mm travel range 1D motorized micromanipulator

  • Special aluminum insert fitting to the microscope stage with a hole and magnetic fixing ring for the 35 mm Petri dish and 80 holes for 10 PCR stripes and a hole for a 32x24 mm2 glass cover slide for testing and high resolution imaging and a hole with a calibration cross-hair

  • CellSorter control unit with 2 high speed fluid valves and USB connection to the computer

  • User friendly intuitive control software for MS Windows OS

  • Accessories, fittings and tubing for liquid control

  • 5 pcs of borosilicate micropipettes for single cell manipulations with calibrated aperture

Motorized Zeiss Axio Observer A1 Inverted Fluorescent Microscope

 

  • Including NA 0.55 LD condensor lens, EC Plan neofluar 10x/0.3 Ph 1 objective lens

  • Accessories for phase contrast and fluorescent imaging

  • Fluorescent illumination system with Prior Lumen 200S metal halide light source

  • Marzhauser 2D motorized microscope stage and motorized focus drive. 

 

Andor Zyla 5.5 Camera

 

  • USB 3.0 sCMOS – Compact low light imaging camera

  • Front-illuminated scientific CMOS sensor

  • 2560 x 2160 pixel array, 6.5 μm pixel size

  • Low read noise of 1.2 e-, sensor stably cooled to 0°C up to an ambient temperature of +35°C

  • Rolling shutter and ‘true’ global shutter modes

  • 12 bit and 16 bit digitization

  • Max. pixel readout rate 2x280MHz

  • iCam, C-mount, USB3.0 interface (interface card and cable included), power supply, trigger cable

 

NE-1000 Syringe Pump

 

  • Holds 1 Syringe up to 60 cc

  • Infusion rates from 0.73 µL/hr (1 cc syringe) to 2100 ml/hr (60 cc syringe)

  • Easy-to-use keypad interface

  • Space Saving Chassis: Foot print size of only 5 3/4" x 8 3/4"

  • Includes all the advanced functions and programming features of the NE-1000 family

Dell Desktop Computer With 23" Dell Monitor

 

  • Processor: Intel Core i7-4790 3,6: 4 Cores, 8M Cache, up to 4.00 GHz

  • Memory: 8 GB DDR RAM

  • HDD: 1000 GB

  • SSD: 120 GB

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